RNA Sequencing of PGE2 or MEHP treated human THP-1 derived macrophages transcriptomes. RNA Sequencing of PGE2 or MEHP treated human THP-1 derived macrophages transcriptomes

THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA) at 25μg/ml for 48h.Then remove PMA and rest for 24h to obtain M0 macropahges. Further applied LPS and IFNγ or IL-4 and IL-13 for 24h to obtain M1, M2 macrophages, respectively. During polarization, cells were treated with prostaglandin E2 or MEHP.Then M1,M2 cells were collected and applied to RNA-sequencing. We found that PGE2 and MEHP significantly impacted metabolic signature of macrophages. Overall design: mRNA profiles of PGE2 or MEHP treated M1 and M2 macrophages were examined by Illumina Hiseq.