Elucidating photoreceptor gene function with in-vivo CRISPR knock-out perturbation assay

NGS-based amplicon sequencing data of guide RNA sequences used in an in vivo perturbation assay in mouse. Cloned guide RNAs were part of two lentiviral CRISPR knock-out perturbation libraries (primary screen library and validation library) which were subretinally injected in transgenic mice expressing the Cas9 endonuclease and relative control mice (no Cas9), as well in a mouse model of retinal degeneration carrying the Rhodopsin P23H variant.