Interactive effects of temperature and food ration on growth and mercury concentration in eastern brook trout

Warming temperatures predicted under global climate change will alter the dynamics of secondary production and trace element accumulation and may interact with environmental factors such as food resource availability to influence growth and bioaccumulation. We conducted laboratory experiments to assess the role of temperature and food ration on Brook Trout growth and mercury (Hg) concentration. Across a range of temperatures (16 – 24°C), Brook Trout growth rate decreased, and Hg concentration increased with temperature. We observed the lowest Hg concentrations in fish under the low temperature (15°C), high ration treatment, and the highest concentrations in the high temperature (22°C), zero ration treatment. The relative growth rank of individual fish was a significant predictor of Hg concentration, with fast-growing individuals having lower Hg. Based on structural equation modelling (SEM), the strongest relationship between Brook Trout Hg and the temperature/ration treatments was throu..., , # Interactive effects of temperature and food ration on growth and mercury concentration in eastern brook trout

Dataset DOI: [10.5061/dryad.v6wwpzh86](10.5061/dryad.v6wwpzh86)

## Description of the data and file structure

This dataset and analysis evaluate the brook trout mercury (Hg) experiments presented by Rutledge et al. 2025. Interactive Effects of Temperature and Food Ration on Specific Growth and Mercury Concentration in Eastern Brook Trout. *CJFAS*. The focus of the laboratory experiment used manipulations of water temperature and food ration treatments to see how Hg concentration changed in brook trout.

### Files and variables

#### File: Rutledge_etal_2025CJFAS_Rproj.zip

**Description:** 

This zipfile contains an RStudio R Project directory. At the top directory level, there are three main files.

1. The first is the R Project file named \"Rutledge_etal_2025CJFAS_Rproj.Rproj\". Open this project file within RStudio.
2. The second and third files are named similarly but re...,