Whole-exome sequencing analysis of patient derived organoids and its parental tumor tissue. Homo sapiens

We assessed whether organoids maintained the gene mutation signatures of their parental tumor. We performed Whole-exome sequencing (WES) analysis in one patient who had diagonsed as rectal adenocarcinoma. Total DNA of organoids and their parental tissues were extracted at day 7 and day 0, respectively. The results show that The tumor-derived organoids were found comprising a highly overlapped (over 97%) single nucleotide variants (SNV) profile with their parental tumor tissue. For the oncogene dataset comprising 731 genes, we found 16 genetic mutations in the P31 organoids and 15 genetic mutations in the parental tumor tissue. It suggests that the organoids recapitulated the genetic information of the parental tumor. .