High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after tumor supernatants induction

The tumor microenvironment (TME) is a unique niche governed by constant crosstalk within and across all intratumoral cellular compartments. In particular, intratumoral high potassium has shown its immune-suppression potency on T cells. However, as a pan-cancer characteristic related to local necrosis, the impact of this ionic disturbance on innate immunity is still unknown. Here, using both murine and human samples, we reveal that intratumoral high potassium leads to profound suppression of the anti-tumor capacity of tumor-associated macrophages (TAMs). We report the high-throughput profiling of the transcriptomes of murine primary BMDMs after tumor conditioned medium (TCM) stimulation. Overall design: Primary BMDMs from C57B6J mice were treated with B16F10 tumor cells conditioned medium supplemented with/without KCl or NaCl for 6 hours, and the final KCl and NaCl concentration was 40mM.