Complexed PIWIL2 binds pre-piRNA

After cleavage by PLD6 at the 5' end, the pre-piRNA is bound by PIWIL2 (HILI, homolog of MILI in mouse), likely in a complex with TDRD1, TDRD12, DDX4 (MVH), ASZ (GASZ), and MOV10L, all of which are required for wild-type levels of piRNA biogenesis. Binding by PIWIL2 is believed to be selective for pre-piRNAs that have uracil residues at their 5' ends.

经过PLD6在5'端的切割，前piRNA被PIWIL2（HILI，小鼠中MILI的同源物）所结合，可能形成与TDRD1、TDRD12、DDX4（MVH）、ASZ（GASZ）和MOV10L等成分的复合体，这些成分对于野生型piRNA生物合成水平是必需的。据信，PIWIL2的结合对具有5'端尿嘧啶残基的前piRNA具有选择性。