Whole-brain transcriptome of Arid1b heterozygote mutation mice age of postnatal day 3

Arid1b is a chromatin remodeler implicated in neurodevelopmental disorders. Arid1b mutant mice with haploinsufficiency (Arid1b HT) displayed persistent excitatory synaptic dysfunction from juvenile to adult stage, decreased synaptic density and transmission. Moreover, they showed autistic-like behaviors in both of early and adult stages, decreased sociability in pup USV calling and adult social interaction, and adult repetitive grooming. To investigate pup stage transcriptomic changes in Arid1b mutant mice, RNAseq analysis of whole brain from wild-type and Arid1b mutant mice at postnatal day 3 was done. Transcriptomic changes support these electrophysiological and behavioral deficits. Arid1b HT mice at postnatal day 3 showed alterations in genes implicated in synaptic functions and ASD. Whole-brain transcriptome of Arid1b heterozygote mutation mice age of postnatal day 3Arid1b是一种参与神经发育障碍发生的染色质重塑因子（chromatin remodeler）。携带单倍剂量不足（haploinsufficiency）突变的Arid1b杂合小鼠（Arid1b HT），从幼年至成年阶段均表现出持续性兴奋性突触功能障碍，突触密度与突触传递功能均显著降低。此外，该模型小鼠在幼年期与成年期均表现出类自闭症行为：幼崽超声发声行为（ultrasonic vocalization, USV）阶段社交能力受损、成年期社交互动表现异常，同时出现成年期重复理毛行为。为探究Arid1b突变小鼠幼年期的转录组变化，本研究对出生后第3天的野生型与Arid1b突变小鼠的全脑组织进行了RNA测序（RNA-seq）分析。转录组学分析结果印证了上述电生理与行为学表型缺陷。出生后第3天的Arid1b HT小鼠中，与突触功能及自闭症谱系障碍（Autism Spectrum Disorder, ASD）相关的基因表达出现显著异常。本数据集为出生后第3天Arid1b杂合突变小鼠的全脑组织转录组。