RNA-Seq JunB WT and KO Tregs Mus musculus

To understand the mechanisms through which JunB regulates Tregs-mediated immune regulation, we examined the global gene expression profiles in the JunB WT and KO Tregs by performing RNA sequencing (RNA-seq) analysis. To study if JunB regulated Treg suppressive function, we created a mouse strain with Treg-specific ablation of Junb (Foxp3YFP-Cre×JunbFlox/Flox) by crossing the Foxp3YFP-Cre mice and the JunbFlox/Flox strain. Non-Treg CD4+ T cells and CD8+ T cells in the KO mice produced a larger amount of IFNγ and TNFα after PMA and ionomycin stimulation, whereas IL-17 production was not influenced. To understand the mechanisms through which JunB regulates Tregs-mediated immune regulation, we examined the global gene expression profiles in the JunB WT and KO Tregs by performing RNA sequencing (RNA-seq) analysis. The subsequent Ingenuity Pathway Analysis (IPA) revealed that the JunB deficiency reduced the expression of a panel of genes reported to be enriched in Tregs.