Transcriptome of Pneumocystis jirovecii during human Pneumocystis pneumonia

We collected a single broncho alveolar lavage from single human patient with Pneumocystis jirovecii pneumonia. Total RNA was extracted using the Ribopure Yeast Kit (Ambion), according to the manufacturer’s instructions. To obtain sufficient amount of RNA, total RNA was amplified using NuGEN Ovation® RNA-seq system and used to build a non-strand-specific library. The library was then sequenced using Illumina HiSeq 2000 technologies.