Effect of deletion of von-Hippel-Lindau (VHL) in the proximal tubule in diabetic kidney disease

To establish the role of proximal tubular hypoxia in diabetic kidney disease, we use a mouse line with a specific deletion of von-Hippel-Lindau (VHL) in the proximal tubule and treat them with streptozotocin (STZ) to induce a type I diabetes mellitus. 10 weeks after induction of diabetes mellitus samples were collected. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of control mice and proximal tubular-specific VHL-deletion mice with and without STZ-treatment (induction of diabetes mellitus).