OVA specific serum antibodies following sensitisation and short or long term challenges

Serum concentrations of OVA-specific antibodies post challenges were assessed by ELISA. In PBS controls no OVA-specific IgG1 or IgG2a was detected and there was a background of OVA-specific IgE of maximally 1.0 IU/ml. Significant differences: *versus respective PBS control, +versus respective time point after short term OVA challenges, */+ p<0.05, **/++ p<0.01, ***/+++ p<0,001. Means±SEM are shown, n≥9 from 3 independent experiments.