Impaired Liver X Receptor alpha phosphorylation affects hepatic chromatin H3K27 acetylation patterns

Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. Besides ligand binding, LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess how changes in the phosphorylation status of LXRα affect the H3K27Ac histone modification, a known marker for active regulatory elements in promoters and enhancers, in livers of mice mice that carry a whole-body phosphorylation deficient-version of LXRα (S196A) compared to wild-type (WT), in response to a high fat-high cholesterol diet. High-throughput profiling of the H3K27Ac histone modification in liver of wild-type (WT) and LXRα-S196A female mice after being fed a High Fat-High Cholesterol diet for 6 weeks.