Genome analysis by next-generation sequencing of potent x Ler descendants

To gain further insight into the root clock mechanism, we performed a mutagenesis screen in plants carrying DR5::Luciferase in combination with markers for subsequent LR organogenesis (pWOX5::ER-YFP and pSCR::ER-GFP12). Out of this screening, we identified a mutant with increased expression of DR5::Luciferase in the OZ and PBS distributed throughout the primary root axis without evident spacing. We further refer to this mutant as potent. Overall design: Approximately 200 seeds generated in the fifth cross of potent x Ler were sown and seedlings with (mutant) and without (Control) potent phenotype were collected and frozen at -80° C. The SNPtrack pipeline was used to identify single variant/nucleotide polymorphisms associated to Ler ecotype as well as all the heterozygous mutations present in the potent sample.