Supplementary Figure 1. DOX induces cardiac and skeletal muscle wasting.

Percentage of body weight change (A), grip strength (B), quadriceps weight to tibia length ratio (MW/TL) (C), percentage change of MW/TL (D), heart weight to tibia length ratio (HW/TL) (E) and percentage change of HW/TL (F) of control (labeled ‘C’) and DOX-treated mice (n=5). (G) Representative image of heart and quadriceps sections from control and DOX mice stained with wheat germ agglutinin (WGA). (H-I) Cross-sectional analysis of myocytes in heart (H) and quadriceps (I) of control and DOX-treated mice. Violin plots show the spread of data with a dashed line representing the median; (n=3). (J-K) Normalized expression of FBXO32 and TRIM63 to Gapdh in cardiac tissue of control and DOX-treated mice (n=5).