BAF60/SWP73 Subunits Define Subclasses of SWI/SNF Chromatin Remodelling Complexes in Arabidopsis

Evolutionary conserved SWI-SNF ATP-dependent chromatin remodeling complex changes nucleosome positioning and chromatin states, affecting gene expression to regulate developmental and hormonal pathways.  We showed the SWP73A and SWP73B - subunits of the SWI/SNF complex - differentially regulate gene expression related to metabolism and hormone biosynthesis, with opposite chromatin occupancy profiles.  The swp73b mutant exhibits hypersensitivity to auxin and salicylic acid (SA). SWP73B inactivation results in SA accumulation, but decreased gibberellin (GA) content and long-term GA treatment partially restored its phenotype.  Both SWP73 subunits are required for maintaining proper diurnal metabolite profiles, with SWP73A regulating essential genes of raffinose and glucosinolate metabolism.  Our study reveals that the duplication of the SWP73 subunit contributes to novel functions of the Arabidopsis SWI/SNF complex in gene transcription. 1. signle-end RNA-seq for 10 libraries, 2 replicas for each genotype. The aim was comparison of transcriptomes of Col-0, swp73a/+, swp73a, swp73b and swp73a/+swp73b