The −122/−69 region is required for optimal FXR-ligand-mediated induction of the SHP promoter in DLD-1, HEK293 and HepG2 cells.

The colon carcinoma (DLD-1), human embryonic kidney (HEK293) and hepatoma (HepG2.rNtcp) cell lines were transfected with the indicated hSHP promoter constructs and expression plasmids for hFXR and hRXRα. Cells were treated with or without 100 µmol/L CDCA (A) or 1 µmol/L GW4064 (B). Luciferase activity was measured to determine SHP promoter activity. Data are presented as means of ± SD; n≥3. Promoter activity in CDCA/GW4064-treated condition is significantly different from the −278/+10 construct in DLD-1 (a), HEK293 (b) or HepG2.rNtcp (c) cells. P≤0.05 in a pairwise comparison by Mann-Whitney U test.