RT-PCR analysis of Vimentin and cancer stem cell surface markers.
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mRNA relative expression of Vimentin (VIM), CD90, CD13, CD133, EpCAM and LGR5 in primary cultures from mixed-intrahepatic (Mixed-IHCCA) or mucin-intrahepatic (Mucin-IHCCA) human cholangiocarcinoma, normalized versus the reference gene GAPDH. mRNA for CD13 was more expressed in mixed-IHCCA while vimentin, CD90, CD133 and LGR5 predominated in mucin-IHCCA. Gene expression was measured in quadruplicate. Mean ± SD of N = 12 experiments. * = p< 0.05. RT-PCR analysis of Vimentin and cancer stem cell surface markers.
创建时间：
2015-11-16
