Bulked sergeant snalysissegregant analysis (BSA) to map peach Rm3

We utilized the bulked segregant analysis (BSA) mapping by sequencing method (Takagi et al., 2013) to map peach Rm3. Briefly, 4 samples (2 parental lines, a resistant pool and a susceptible pool) were used for high-throughput specific length amplified fragment sequencing (SLAF-Seq), and about 265.9 million high-quality short reads were generated. The average read depth was 74-fold in 01-77-03, 90-fold in CN13, 50-fold in the resistant pool and 71-fold in the susceptible pool (Table S1). Among the 910,069 polymorphic markers identified, 194,196 single-nucleotide polymorphisms (SNPs) and 38,497 insertion/deletion polymorphisms (Indels) were filtered out and identified between the 2 bulked pools (Table S2). The SNP index was calculated by integrating the single-nucleotide polymorphisms of the 2 pools.