Real-time quantitative PCR analysis of lungs from Litomosoides sigmodonis-infected mice. Real-time quantitative PCR analysis of lungs from Litomosoides sigmodonis-infected mice

A pool of cDNA from naive BALB/c mice lungs (n=8) was compared with one from D70 p.i. Litomosoides sigmodontis-infected mice (n=8) by profiling 84 cytokine-related genes simultaneously. Screening of inflammatory lung environment was performed with a qRT-PCR array (Mouse Cytokines & Chemokines RT2 Profiler PCR Array, Qiagen, Germany) according to manufacturer’s instructions. Overall design: qPCR gene expression profiling. Equal amounts of total RNA per mice were used in the assay.