Next Generation Sequencing Facilitates Quantitative Analysis of Changed MicroRNAs in Psoriasis Patients.

Purpose: MicroRNAs are small noncoding RNAs involved in autoimmune diseases. The goals of this study are to identify changed miRNAs in the peripheral blood mononuclear cell (PBMC) of psoriasis patients. Results: Gene ontology analysis showed that obviously changed genes enriched in autoimmune disease related pathways, suggesting miR-148a is important in autoimmune responses. 990 protein-coding genes upregulated in their mRNA abundance in miR-148a-/- monocytes on day 0, and 1036 genes in miR-148a-/- monocytes on day 3. Among them, 136 genes were found upregulation on both day 0 and 3 in miR-148a-/- monocytes. Peripheral blood mononuclear cells (PBMC) were isolated from healthy control (HC) and psoriasis patients (P) by using Ficoll . Then, miRNA libraries were prepared by using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA) and then purified by QIAquick® PCR Purification Kit (Qiagen, Hilden, Germany), and put to size selection by using gel Prep. The Library quality was determined by Bioanalyzer 4200 (Agilent, Santa Clara, CA, USA).The GO analysis on differentially expressed genes was performed with an R package: Clusterprofiler using a p<0.05 to define statistically enriched GO categories.