Purification of recombinant hTyrCtr and two temperature sensitive variants from 10 g of larval biomassa.
收藏Figshare2015-12-02 更新2026-04-29 收录
下载链接：
https://figshare.com/articles/dataset/_Purification_of_recombinant_hTyrC_tr_and_two_temperature_sensitive_variants_from_10_g_of_larval_biomass_a_/893178
下载链接
链接失效反馈官方服务：
资源简介：
a The total protein was estimated by the Warburg – Christian method using absorbance at 260/280 nm (Sigma-Aldrich); L-DOPA activity was determined as described in Materials and Methods section. Specific activity was obtained as the L-DOPA enzyme activity multiplied by the sample total volume and divided by total protein. The tyrosinase content of hTyrCtr, R422Q and R422W in protein extracts were estimated from SDS-PAGE gels as 100% X tyrosinase band intensity/total protein band intensity.
创建时间：
2015-12-02
