Figure10a-d.nd2

Figure 10. S. aureus MRSA biofilms grown without P. aeruginosa cell-free conditioned media (CFCM) (A – D) or with CFCM (E – H) for 24 hours to assess the viability of bacterial cells re-maining. Wheat Germ Agglutinin Alexa Fluor 647 was used to identify S. aureus cells (A and E). Live (B and F) and Dead (C and G) were visualized using SYTO 9 and Propidium Iodide, respec-tively, and captured simultaneously. Merged images were constructed by overlaying all channels to visualize the extent to which S. aureus cells were killed by CFCM (D and H). Viability was determined using ImageJ Biofilm viability checker[29].