Effect of acute embryonic exposures to tris(4-chlorophenyl)methane and tris(4-chlorophenyl)methanol in the zebrafish

Tris(4-chlorophenyl)methane (TCPM) and tris(4-chlorophenyl)methanol (TCPMOH) are two marine pollutants of emerging concern, found in marine mammals and coastal bird populations. Here, we assess the response to these compounds due to acute exposures in zebrafish embryos (from 96-100 hours post fertilization). We analyzed a total of 15 samples: Samples 1-5 were controls, exposed to 0.01% v/v dimethyl sulfoxide for 4 hours between 96-100 hours post fertilization. Samples 6-10 were exposed to 50 nM TCPM, and Samples 11-15 were exposed to 50 nM TCPMOH. Samples were collected from 3 experimental replicates, with randomized embryos acquired from 20 breeding pairs.