Whole exome sequencing of Men1 KO mice
收藏科学数据银行2025-09-01 更新2026-04-23 收录
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资源简介：
Mice harboring TAM-inducible Men1f/f;UBC-Cre alleles were induced with 100 mg/kg TAM daily for five consecutive days at 4-6 weeks to generate Men1∆/∆ models, with normal lung tissue from Men1f/f controls, tumor-adjacent normal tissue, and tumor tissue from 12-month post-TAM Men1∆/∆ mice subjected to whole-exome sequencing by Novogene (Beijing, China) through Covaris M220 shearing (180-280 bp), Agilent SureSelect Human All Exon V5/V6 library preparation (end repair/phosphorylation/adenylation/adapter ligation), RNA bait hybridization (543,872 baits targeting 334,378 exons in 20,965 genes), and Illumina HiSeq sequencing, followed by bioinformatic analysis using BWA(v0.1.22)/Samblaster for alignment, Sambamba(v0.4.7) for duplicate marking, SAMtools(v1.0) for SNP/INDEL detection, muTect(v1.1.4)/Strelka(v1.0.13) for somatic SNV/INDEL, control-FREEC(v6.7) for somatic CNV, ANNOVAR(2013Aug23) for annotation, and MuSic(v0.04) for mutational signature/pathway enrichment.
提供机构：
Aobo Lian; Guanghui Jing
创建时间：
2025-07-16
