Gene expression profiling of FACS-purified striatal medium spiny neurons in Huntington's disease model mouse

Gene expression profiling of purified medium spiny neurons (MSNs) in R6/2 Huntington’s disease (HD) model mouse. To purify MSNs by FACS, R6/2 was crossed with Scn4b-Venus transgenic mouse expressing Venus in MSNs. MSN gene set excluded gene expression alterations in other neuronal populations, indicating the gene set displays MSN-specific pathological changes in HD. We identified the dysregulated genes expression, which are associated with apoptosis and transcriptional regulation, and found considerable number of disease causative genes in MSN gene set. Purified-MSNs were obtained from 4-week-old male R6/2;Scn4b-Venus and control mouse. The following number of replicates was analysed for each genotype: 4 for R6/2;Scn4b-Venus, 4 for WT;Scn4b-Venus.