PEDV N interactiome _Label-Free.xlsx

Porcine epidemic diarrhea virus (PEDV) is the agent of PED which causes numerous economic losses for the swine industry each year. Till now, no effective vaccine or drugs are available to contain this disease. As a result, it is critical urgent to elucidate the PEDV interactome. The nucleocapsid (N) of PEDV plays a key role in viral replication. In this study, the N gene was cloned into pEGFP-C1 and the recombinant plasmid pEGFP-N was prepared. In order to obtain the interactome of N protein, the plasmids pEGFP-N and pEGFP-C1 were transfected in 293T and the cellular binding partners were pulled down using the GFP-trap. These proteins were identified by label-free MS. The results showed that approximately 1200 cellular proteins were initially identified and quantified, which represented both specific and nonspecific interactions. Under the criteria of more than 2 unique peptides, a false discovery rate (FDR) ≤ 1%and a p value &lt;0.05 for the t-test analysis, 147cellular potential proteins were identified (as listed in the table). <b></b><i></i><br>