Steady-state kinetic parameters of mAOX3 wildtype and mAOX3 variants containing single amino acid exchanges in the active site.
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µM DCPIP was used as terminal electron receptor.1 100 2 Molecular oxygen in air saturated buffer was used as terminal electron acceptor.°C; kcat was corrected for molybdenum content3 Determined in 50 mM Tris (HCl) pH 8.00 at 37[23] and corrected for molybdenum content.4 Values from n.d.: non detectable
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2015-12-02
