Morphogenetic and chemical stability of long-term maintained <i>Agrobacterium</i>-mediated transgenic <i>Catharanthus roseus</i> plants

Transgenic <i>Catharanthus roseus</i> plants (transgenic Dhawal [DT] and transgenic Nirmal [NT]) obtained from the <i>Agrobacterium tumefaciens</i> and <i>Agrobacterium</i><i>rhizognenes</i>-mediated transformations, respectively, have been maintained <i>in vitro</i> for 5 years. Plants were studied at regular intervals for various parameters such as plant height, leaf size, multiplication rate, alkaloid profile and presence of marker genes. DT plant gradually lost the <i>GUS</i> gene expression and it was not detected in the fifth year while NT plant demonstrated the presence of genes <i>rol</i><i>A</i>, <i>rol</i><i>B</i> and <i>rol</i><i>C</i> even in the fifth year, indicating the more stable nature of <i>Ri</i> transgene. Vindoline content in the DT was two times more than in non-transformed control plants. Alkaloid and tryptophan profiles were almost constant during the 5 years. The cluster analysis revealed that the DT plant is more close to the control Nirmal plant followed by NT plant.分别通过根癌农杆菌（*Agrobacterium tumefaciens*）与发根农杆菌（*Agrobacterium rhizogenes*，原文笔误为rhizognenes）介导转化获得的转基因长春花（*Catharanthus roseus*）植株——转基因达瓦尔（DT）与转基因尼尔马尔（NT），已通过离体（in vitro）培养维持5年。研究人员定期对植株的多项指标进行检测，包括株高、叶面积、繁殖速率、生物碱谱（alkaloid profile）及标记基因存在情况。DT植株的GUS基因（GUS）表达逐渐丧失，至培养第5年时已无法检测到该基因的表达；而NT植株在第5年仍可检测到*rolA*、*rolB*与*rolC*基因的存在，表明Ri转基因（Ri transgene）具有更稳定的遗传特性。DT植株的文多灵（vindoline）含量为非转化对照植株的2倍。培养5年期间，植株的生物碱谱与色氨酸谱（tryptophan profiles）基本保持稳定。聚类分析（cluster analysis）结果显示，DT植株与对照尼尔马尔植株的亲缘关系更为接近，其次为NT植株。

搜集汇总

背景与挑战

背景概述

该数据集研究了通过农杆菌介导转化的转基因长春花植物的长期形态发生和化学稳定性，在5年体外培养期间，分析了植物高度、生物碱谱和标记基因等参数。结果显示，DT植物逐渐失去GUS基因表达，而NT植物中的rol基因更稳定，且DT植物的长春多灵含量高于对照，生物碱谱保持基本恒定。