Replication Data for "The Effects of Green Tea and/or Licorice Aqueous Extracts on Hepatic Oxidant/Antioxidant System of Dimethylnitrosamine-Intoxicated Male Albino Rats."

Description of data 1. Effects of the tested materials on hepatic content of total thiols Table 6 demonstrated that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). The combination of green tea and licorice increased the hepatic content of total thiols by 18.03 % when it was compared with normal control, while green tea or licorice had no significant change. DMN decreased significantly that content by 22.40 %, while green tea, licorice or the combination of both antagonized the bad effect of DMN by increasing that content significantly by 38.73 %, 13.38 % and 33.10 % respectively as shown in table 1. 2. Effects of the tested materials on hepatic content of reduced glutathione (GSH) Table (6) revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Licorice or green tea increased significantly the hepatic content of GSH in relation to normal control by 56.60 % or 35.85 % respectively, while the mixture decreased that level significantly by 20.75 %. On the other hand, DMN caused a profound increase in hepatic content of GSH relative to normal control by 64.15 %. Licorice or green tea extract administration to DMN-injected animals increased that content by 72.41 % or 22.99 % respectively while the mixture decreased that content insignificantly as shown in table 1. 3. Effects of the tested materials on hepatic activity of glutathione reductase (GSHr) Table 6 depicted that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Licorice, green tea or the combination decreased significantly the hepatic activity of glutathione reductase in relation to normal control by 25.60 %, 13.60 % or 14.40 % respectively, while DMN significantly increased the activity by 12.00 %. Green tea, licorice, or the mixture antagonized the stimulating effect of DMN by decreasing that activity by 30.71 %, 20.00 % or 34.29 % respectively as revealed in table 2. 4. Effect of the tested materials on hepatic of total glutathione-S-transferase (GST) activity Table 6 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea, licorice or the mixture caused significant decreases in the hepatic activity of GST relative to normal control by 10.79 %, 26.75 % or 14.24 % respectively, while DMN exerted a significant increase in that activity by 17.37 %. Green tea, licorice or the mixture exerted significant decrease of the elevated hepatic GST activity of DMN-administered animals by 35.85 %, 9.65 % or 31.07 % respectively as shown in table 2. 5. Effect of the tested materials on hepatic of total glutathione peroxidase (GSHpx) activity Table 6 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea exerted an insignificant change in the GSHpx activity, while licorice or the mixture produced significant decreases by 25.52 % or 12.74 % respectively when compared with normal control. On the other hand, DMN caused a significant increase by 11.79 % relative to normal control. Green tea, licorice or the mixture administration to DMN-injected animals caused significant decreases in that activity by 30.38 %, 12.66 % or 27.43 % respectively as shown in table 2. 6. Effects of the tested materials on hepatic of total superoxide dismutase (SOD) activity Table 7 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea or licorice decreased significantly the hepatic activity of SOD in relation to normal control by 86.56 % or 62.26 % respectively, while the combination increased significantly that activity by 19.20 %. DMN decreased significantly that activity by 17 %. Green tea or the combination did not affect the lowering effect of DMN, but enhances insignificantly the SOD activity. On the other hand, licorice profound the lowering effect of DMN by decreasing that activity by 15.37 % as represented in table 3. 7. Effects of the tested materials on hepatic activity of catalase Table 7 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea, licorice or the combination decreased the hepatic activity of catalase by 27.70 %, 10.14 or 30.41 % respectively, while DMN increased significantly that activity by 28.38 % relative to normal control. Green tea, licorice or the mixture attenuated the stimulating effect of DMN by decreasing the catalase activity by 48.79 %, 20.53 % or 8.42 % respectively relative to DMN-injected control rats as shown in table 3. 8. Effects of the tested materials on hepatic content vitamin C Table 7 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.01). All the treated compounds either the sole drinking or the mixture exerted no significant changes on hepatic content of vitamin C in relation to normal control. Although DMN had also no effect on that content, but it insignificantly increased that content. Green tea, licorice or the mixture administration to DMN-injected animals decreased that content by 14.81 %, 15.74 % or 10.19 % respectively as revealed in table 3. 9. Effect of the tested materials on plasma level of uric acid Table 7 demonstrated that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). As shown in table 3. Green tea, licorice, DMN or the mixture induced significant increases in plasma uric acid relative to control by 5.0 %, 15.0 %, 15.0 % or 7.50 % respectively. On the other hand, licorice extract administration induced no significant change of plasma uric acid of DMN injected rats while green tea or the combination antagonized the effect of DMN by decreasing that level by 10.86 % or 8.70 % respectively. 10. Effects of the tested materials on hepatic content of thiobarbituric acid reactive-substances (TBARs) Table 8 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea had no effect on hepatic level of TBARs compared to normal control, while licorice or the mixture of licorice and green tea increased that level by 53.36 % or 61.34 % respectively. DMN caused vigorous increase in TBARs level by 173.11 % in relation to normal control. Green tea, licorice or the combination of both decreased the elevated TBARs levels exerted by DMN by 16.62 %, 23.23 % or 37.54 % respectively as shown in table 4. 11. Effects of the tested materials on hepatic content of nitrite Table 8 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001) While the mixture caused a significant increase in hepatic content of nitrite by 25.47 %, green tea or licorice decreased that content significantly by 34.05 % and 6.68 % respectively when they were compared with normal control. DMN caused a significant decrease in that content by 11.35 % when it was compared with normal control. Green tea or the mixture attenuated the effect of DMN by increasing that content by 50.64 % or 10.95 % respectively relative to DMN-injected control rats, while licorice enhanced the DMN effect by decreasing the nitrite content by 33.29 % as shown in table 4. 12. Effect of the tested materials on plasma CA 19.9 level Table 8 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). As represented in table 4, DMN caused three fold increase in plasma CA 19.9 level by 202.38 %, although the tested extracts exerted insignificant increase in that level when compared to normal treated rats. On the other hand, the mixture, licorice or green tea caused significant decrease in plasma CA 19.9 level of DMN-injected rats by 59.84 %, 50.39 %, or 33.73 % respectively. 13. Effect of the tested materials on hepatic content of collagen Table 8 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). Green tea, licorice or the mixture caused significant decreases in the hepatic content of collagen by 6.02 %, 19.42 % or 20.56 % respectively relative to normal control, while DMN caused a significant increase by 5.19 %. Although licorice exerted a significant increase in the content of DMN-injected animals by 13.23 %, green tea or the mixture caused significant decreases by 30.80 % or 15.30 % respectively as shown in table 5. 14. Effect of the tested materials on hepatic content of elastin Table 8 revealed that the general effect, in between groups, was found by one way ANOVA to be very highly significant (P 0.001). DMN increased the hepatic content of elastin significantly by 16.47 %, while green tea, licorice or the mixture exerted significant decreases by 8.03 %, 19.68 % or 11.24 % respectively relative to normal control. Green tea, licorice or the mixture caused significant decreases of elastin content of DMN-intoxicated rats by 32.76 %, 4.83 % or 22.76 % respectively as shown in table 5.