Proportion of N. pubens field-collected colony samples producing an amplicon with oligonucleotide primers (see Table 1) designed to each sequence indicated.

In each column, the percentage of samples and the total number evaluated [n] yielding an amplicon are provided.aFor positive strand viruses, the forward tagged primer was used to detect the negative (or replicative) viral genome strand. Similarly, for negative strand viruses the reverse tagged primer was used to detect the positive (or replicative) viral genome strand.bAll (100%) of these field samples yielded an amplicon when either the forward or reverse oligonucleotide primer was used in cDNA synthesis suggesting the presence of ambisense RNA.