DAS-40278-9 OF EVENT AAD-1， TRANSGENIC LINES OF CORN and RELATED IDENTIFICATION EVENTOESPEC??FICO OF THE SAME

The present are in part relates to the breeding of plants and plants tolerant to herbicides. The present includes one event of transformation of aad-1 in plants of corn comprising one sequence of polynucleotides that painted in the present， inserted in one specific site within the genome of one cell of maize. In some forms of embodiment， such event/sequence of polynucleotides are may ' stacking " with other characteristics， which include， by example， other (s) gene (is) with tolerance to herbicides and/or of insect inhibitory proteins. Additionally， present in the subject invention provides assays for detecting the presence of event in question in one sample (by example， of grain of corn). The assays may be based in the of DNA sequence of the recombinant construct， inserted in the genome of maize， and in the genomic sequences that flanking the site of insertion. Also provided are the kits and conditions useful for the to carry out the tests. Claim 1: One method for detecting the DNA of maize event DAS-40278-9 that comprising SEQ ID no: 29 is the one which shows DNA comprising of corn， characterized in that said method Comprising: i) contacting said sample with one first primer which are bonded to one flanking sequence of event， said flanking sequence selected from the group that consists in the nucleotides 1 - 1873 of the SEQ ID no: 29， the nucleotides 6690-8557 of the SEQ ID no: 29 and its full complement; and one second primer which are bonded to the sequence of insertion of Event， wherein said sequence of insertion the residues 1874-6689 of the SEQ ID no: 29 or their complement; subjecting said sample to the in chain reaction of the polymerase; And testing one amplicon generated between said primers， or ii) put in contact with one said sample polynucleotide comprising an sequence which selected from the group that consists in the residues 1863 to 1875 Of the SEQ ID no: 29， the residues 6679 to 6700 of the SEQ ID no: 29， and their complements; in which the said method further comprising subjecting said sample and said polynucleotide to conditions of stringent hybridization; And assaying said sample for the hybridization of said polynucleotide to said DNA.