SYSTACT CyTOF dataset associated with Multiomics approaches disclose very-early molecular and cellular switches during insect-venom allergen-specific immunotherapy: an observational study

This dataset contains all the raw mass cytometry (CyTOF) fcs files associated with Pogorelov et al. 'Multiomics approaches disclose very-early molecular and cellular switches during insect-venom allergen-specific immunotherapy: an observational study', Nature Communications, 2024, in Press. The dataset contains all the raw fcs files generated by CyTOF analysis of 199 time-series samples including ~200 million of deeply characterized immune cells, either from patients with insect-venom allergy or patients with pollen allergy following allergen-specific immunotherapy (AIT) or from healthy controls (HC), who did not receive any AIT or other immunotherapy. As specified in the manuscript, we sampled different individuals up to seven times (0h, 8h, 24h, Day 7, Week 2, Week 6 and Week 12) over a three-month period. All the samples from insect-venom allergy patients are stored in the zipper folder called "Venom" and all the samples from pollen allergy patients are in the zipper folder called "Pollen" while all the samples from HC are placed in the zipper folder called "Control". We also provide the following annotation files (metadata) to help the readers to better understand, validate and re-analyze our immunological dataset.

1, In addition to the Supplementary Tables in our manuscript, we also attached one Word table file mapping the 36 specific metal isotypes and the specific Ab target markers.

2, The Excel file with the list of 199 samples, showing the correct group and time point information (of note, the manually input sample information in the file name might be wrong for some samples). The correct time point information is in the column "Timepoint Detail" and "Timepoint Final".

Associated with these raw fcs files, our processed results of all the CyTOF data are deposited in another webpage. Our large-scale interactive interlinked immunological Data resource (i3Dare) allows investigators to more effectively explore or reuse our immunological datasets of each immune subset from each participant at each time point (please refer to https://public.tableau.com/app/profile/lihpublicdata/viz/i3Dare_SYSTACT_Database/SYSTACTHome). OF note, to further protect privacy, the pseudoIDs within groups in i3Dare were re-labelled again. 

Please read the accompanying information/metadata files, our main manuscript and Supplementary Information, our processed data on the i3Dare webpage and the published Source Data together with the raw fcs files in order to reproduce our published results.