RNA sequencing to analyze trasncriptional profiles and screen potential regulators in confined migration

To identify potential regulators involved in confined cell migration, we performed transwell migration assay with 3 µm pore size to simulate native physical confined microenvironment. Transmigrated cells were collected for RNA sequencing to analyze transcriptional profile changes in comparison to parental cells. Overall design: Here are two types of samples, namely control and 3 µm_confine. The control group represents parental MEF cells. The 3 µm_confine group represents MEF cells transmigrated through transwell with 3 µm pore size.