lin-42 mutations suppress heterochronic phenotypes of multiple heterochronic miRNAs.

aAnimals contain maIs105, an adult-specific col-19pro::GFP reporter that is integrated on chromosome V.bPresence and quality of cuticular alae structures were assayed by Normarski DIC optics. Only one side of each animal was scored.For most alleles of lin-42, L4-staged animals expressed adult alae but alae were not continuous (contained gaps) along the entire lateral.cAnimals harboring the alg-1(ma192) mutation display a 100% vulval bursting phenotype. The lin-31(n1053) mutation suppresses the vulval bursting phenotypes and causes lin-31(n1053); alg-1(ma192) animals to display a 100% vulvaless (Vul) phenotype. The lin-31(n1053) allele does not alter the seam cell heterochronic phenotypes of alg-1(ma192).dlin-2(e1309) mutations cause animals to lack a vulva but do not alter temporal patterning of the hypodermal seam cells. The lin-2(e1309) allele was included in strains that contain let-7 mutations to prevent the highly penetrant vulval bursting phenotypes.