Summary of results from the experimental evaluation of predator DNA removal techniques for the PCR amplification of the semi-digested prey homogenate in the gut contents of Neocirrhites armatus (n = 10) and Paracirrhites arcatus (n = 10).

Two versatile primer sets (“COI”, Folmer et al., 1994; and “dgCOI”, Meyer, 2003) and two predator DNA removal strategies (restriction enzymes and blocking primers) were used. As the primer set “COI” (Folmer et al., 1994) does not enable amplification of the COI region of N. armatus, no predator DNA removal was required (see results of preliminary tests for host DNA co-amplification).