Downregulation of MED12 via siRNA in prostate cancer cell lines (3' tag DGE)

The Mediator complex is a multi-subunit protein that modulates gene expression on a genome-wide scale. MED12 and cyclin-dependent kinase 8 (CDK8) or its paralog CDK19 are components of its kinase module that regulate the proliferation of prostate cancer cells. In this study, we investigated how MED12 and CDK8/19 influence cancer-driven processes in prostate cancer cell lines, focusing on AR activity and enzalutamide resistance. Overall design: To investigate the relevance of MED12 in enzalutamide sensitive and resistant prostate cancer cells, we performed siRNA mediated knockdown and analyzed the response using high-throughput 3' tag differential gene expression analysis.