Spatial proximity of DNA breaks and formation of structural variations

In this study, we set out to quantify the impact of spatial proximity on structural variant (SV) formation by targeting borders of highly conserved topologically associated domains with double-stranded breaks (DSBs) induced by CRISPR, comparing the results to size-matched non-interacting loci. Furthermore, we investigated whether spatial proximity between DSBs induced by CRISPR pairs would affect SV formation kinetics and characteristics of the chimeric SV junctions.