Sulfur spring sediment and crust microbial communities metatranscriptome

A comparative metatranscriptomic study was performed at a sulfur spring (Zodletone) in Soutwestern Oklahoma along distance and temporal gradients. Total RNA was extracted from 10 samples, and mRNA was enriched and amplified. These steps were followed cDNA synthesis and library construction, by pyrosequencing (454, Titanium chemistry). Transcript analysis will be used to reveal the functions occuring along these gradients (e.g. sulfur oxidation and reduction). As well, we plan to uncover the functional roles of novel bacterial candidate divisions found at the site (e.g. OP11) by comparing the metatranscriptomes generated in this study to genomes and/or partial genomes of these novel candidate divisions. Description: Site Location: Near Mountain View, OK Site coordinates: 35.100293, -98.749601 Altitude: 408 m Collection date: 11/5/2009