BIOGRID CURATED DATA FOR NCL1 (Saccharomyces cerevisiae (S288c))

Protein-Protein, Genetic, and Chemical Interactions for NCL1 (Saccharomyces cerevisiae (S288c)) curated by BioGRID (https://thebiogrid.org); DEFINITION: S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120

蛋白质-蛋白质、遗传和化学相互作用数据集，针对NCL1（酿酒酵母S288c）由BioGRID（https://thebiogrid.org）整理；定义：依赖于S-腺苷-L-蛋氨酸的tRNA：m5C甲基转移酶；在tRNA和含内含子的前tRNA中多个位置甲基化胞嘧啶至m5C；在响应过氧化氢时，增加携带m5C摆动位置tRNALeu(CAA)的比例，导致富含TTG密码子的基因mRNA的选择性翻译；NCL1的丢失赋予对氧化应激的超敏感性；与Nop2p及与人类增殖相关的核仁蛋白p120相似。