MOESM2 of Systematic quantitative analysis of H2A and H2B variants by targeted proteomics

Additional file 2. Validation criteria for each signature peptide. Peptide number, protein names, peptide sequence, ESP predictor score, and length of peptides are indicated. The criteria detailed in the results sections (Theoretical histone peptides relevant for a targeted proteomic analysis and Trypsin missed cleavages in signature peptides) were also reported for each peptide. They include their status as fully tryptic or non-tryptic (e.g., C-terminal of the histone variant), if their length is within 6–23 amino acids, if they are not chemically modified, and if the monitored peptides have no neighboring K/R residue.