Table 1 in Phylogeny and classification of jumping plant lice of the subfamily Liviinae (Hemiptera: Psylloidea: Liviidae) based on molecular and morphological data

Table 1. Primers and conditions for PCR.
GenePrimer setPrimer sequence (5'-3')Amplicon size (bp)PCR conditionsRound in nested PCRReferencesCOImtd6-FGGAGGATTTGGAAATTGATTAGTTCC472PCR: 94°C for 3 min; 40 cycles of 92°C for 30 sFirstSimon et al. 1994, Percy et al. 2018mtd9-RCCCGGTAAAATTAAAATATAAACTTC55°C for 30 s and 72°C for 1 min; 72°C for 10 minFirstdoa_CO1_F1TMATTGAYCAAGGAGTAGGWACNested PCR: 94°C for 3 minSecondthis studydoa_CO1_F2CAAGGAGTAGGWACAGGRTGAAC20 cycles of 92°C for 30 s, 60°C for 20 s (descrease by 0.5°C/ cycle) and 72°C for 1 minSeconddoa_CO1_ R1AGTAGTATAGTGATAGCTCCAGC20 cycles of 92°C for 30 s, 54°C for 20 s and 72°C for 1 min; 72°C for 10 minSeconddoa_CO1_ R2ATTGCTCCTGCTAATACTGGAAG94°C for 3 min; 40 cycles of 92°C for 30 s, 59°C for 30 s and 72°C for 1 min;Second72°C for 10 minCytbcytBfTGAGGNCAAATATCHTTYTGA385PCR:94°C for 3 min; 40 cycles of 92°C for 30 sFirstTimmermans et al. 2010, Percy et al. 2018cytBrGCAAATARRAARTATCATTCDG53°C for 30 s and 72°C for 1 min; 72°C for 10 minFirstdoa_cytb_F1ACTTGACTATGRGGAGGRTTTGNested PCR:94°C for 3 minSecondthis studydoa_cytb_F2ACTATGAGGAGGGTTCGCAGTTG20 cycles of 92°C for 30 s, 58°C for 20 s (descrease by 0.5°C/ cycle) and 72°C for 1 minSeconddoa_cytb_R1GGRGTTCTTATAGGATTGGC20 cycles of 92°C for 30 s, 52°C for 20 s and 72°C for 1 min; 72°C for 10 minSecond94°C for 3 min; 40 cycles of 92°C for 30 s, 57°C for 30 s and 72°C for 1 min72°C for 10 minH3H3a-FATGGCTCGTACCAAGCAGAC(ACG) GC350PCR:94°C for 3 min; 40 cycles of 92°C for 30 sFirstColgan et al. 1998H3a-RATATCCTT(AG)GGCAT(AG)AT(AG) GTGAC53°C for 30 s and 72°C for 1 min; 72°C for 10 minFirstH3_newFTCNACCGGAGGNAARGCTCNested PCR:94°C for 3 minSecondthis studyH3_newRATGATRGTNACWCGCTTGGCG20 cycles of 92°C for 30 s, 58°C for 20 s (descrease by 0.5°C/ cycle) and 72°C for 1 min;Second20 cycles of 92°C for 30 s, 52°C for 20 s and 72°C for 1 min; 72°C for 10 min94°C for 3 min; 40 cycles of 92°C for 30 s, 58°C for 30 s and 72°C for 1 min72°C for 10 minwgPswgFACATGYTGGATGAGAYTACCA268PCR:94°C for 3 min; 30 cycles of 94°C for 30 sFirstHall et al. 2016PswgFTCTTGTGTTCTATAACCACGCCCAC58°C for 30 s and 72°C for 1 min; 72°C for 10 minFirstwg_F2ARGATCGATTTGAYGGTGCKTCTCNested PCR: 94°C for 3 minSecondthis study