five

0549_2 Fission yeast Pab1 interactome

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NIAID Data Ecosystem2026-05-02 收录
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https://www.omicsdi.org/dataset/pride/PXD047697
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We performed mass-spectrometry analysis of proteins co-purifying with the Pab1, the B55 regulatory subunit of the PP2A protein phosphatase complex. Pab1 was tagged with YFP at the C-terminus and expressed from its endogenous promoter at its chromosomal locus. After one hour of nitrogen starvation, Pab1-YFP was pulled down and co-purifying proteins were analysed by mass-spectrometry. Our results revealed the presence of all components of PP2A protein complexes including Paa1, the catalytic subunits Ppa1, Ppa2, and Ppa3.
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2024-12-02
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