Amino acid residues at VP1-98 and VP1-145 of EV71 variants in postmortem tissue samples.

++; Positive by both virus isolation and molecular detection.
+; Virus isolation negative but positive by molecular detection.
±; Virus isolation negative but positive by molecular detection with a weak signal.
−; Negative for both virus isolation and molecular detection.
“KE” represents deduced amino acid residues at VP1-98K and VP1-145E. “KE/EE” indicates that mixed nucleotides [K(AAA) and E(GAA)] were identified at VP1-98 on sequencing chromatogram obtained from the clinical samples by direct RT-PCR. n.d.; the sequences could not be determined.
*The sequence results were determined from the partial VP1 region obtained by the CODEHOP RT-PCR. Amino acid substitutions from the original inoculated viruses are indicated in bold. Amino acid substitutions other than VP1-98 and VP1-145 in the entire or partial VP1 sequence were indicated as K242Q, or K242T, etc.
Amino acid residues at VP1-98 and VP1-145 of EV71 variants in postmortem tissue samples.