mixture contained meat from S. domesticus (pig) and G. gallus (chicken) in the following proportions, 10:0, 8:2, 6:4, 4:6 , 2:8, and 0:10. Raw sequence reads

Accurate and efficient analytical methods that have been developed are mostly based on the detection of species on the DNA level by PCR. However, PCR-based methods have an inherent problem of amplification bias, whereas whole-genome sequencing (WGS) methods are difficult to implement. In this study, we tested the performance of four markers, namely, mitochondrial genome (mitogenome), COX1, 16S rRNA, and 18S rRNA genes, in the detection of species from mixtures containing multiple taxon compositions by using WGS directly.