Human cochlear gene expression

Transcriptome analysis of human inner ear tissue Hearing loss is common and caused by a wide range of molecular and cellular pathologies. Current diagnosis of hearing loss depends of a combination of physiologic testing, patient history and in some cases genetic testing. Currently no biopsy or equivalent procedure exists to diagnose inner ear disorders. The goal of this study was to determine if miRNAs could be identified in human perilymph potentially leading to the development of biomarkers for inner ear disease. Analysis of miRNAs was carried out by evaluating miRNA targets in a cochlear transcriptome library (GSE128505) derived from human inner ear tissue harvested during surgery in which the inner ear is removed. Basilar membrane, cochlear lateral wall and modiolous harvested at time of transcochlear approach to skull base meningioma Total RNA isolated and analyzed with Affymetrix Human Transcriptome 2.0 array; Data was processed with the Affymetrix console