Electrophysiology of neurons adjacent and away from microglia in control and TBI conditions

Aim: We investigated whether neurons adjacent to satellite microglia (microglia juxtaposed to neurons with their soma and processes entwining around the neuronal soma) have differences in intrinsic excitability and how this relationship might change after traumatic brain injury (TBI) or with antagonism of the P2Y12 receptor.
Methods: Whole cell patch clamp experiments were performed on in vitro slices of the orbitofrontal cortex (OFC) in sham or TBI conditions or incubation with a P2Y12 receptor antagonist (PSB 0739 1microM) and a series of current steps was used to invoke action potentials. Tmem119 mice (Jackson Laboratory 031823) were utilized to identify GFP+ microglia and determine whether a neuron was adjacent to a satellite microglia or away. TBI was induced with a controlled cortical impact ~1-2 months before testing. Full details are provided in Feichtenbiner et al. (J Neuroinflammation. 2025. 22:9.)
Results: Satellite microglial association reduced the intrinsic excitability of..., , # Electrophysiology of neurons adjacent and away from microglia in control and TBI conditions

Dataset DOI: [10.5061/dryad.ns1rn8q5p](https://doi.org/10.5061/dryad.ns1rn8q5p)

## Description of the data and file structure

The response to a series of current steps was recorded with pClamp software (Molecular Devices) for every cell. Files are stored in the .abf format. The Metadata file provides the filename associated with each cell and the associated sham, TBI or drug condition as well as the sex of the mouse.

## Code/software

Abf files can be opened in pClamp software such as Clampfit which is free to download as well as other programs such a Matlab with easily accessible conversion scripts available online.

## Access information

Data was derived from the following sources:

* Feichtenbiner AB, Sytsma K, O'Boyle RP, Mittenzwei R, Maioli H, Scherpelz KP, Child DD, Li N, Ariza Torres J, Keene L, Kirkland A, Howard K, Latimer C, Keene CD, Ransom C, Nolan AL. Satellite microglia: ma...,