Role of PML in ISG induction in primary postnatal sympathetic neurons

A previous study has shown that PML-dependent recruitment of HIRA to ISG promoters contributes to the up-regulation of gene expression as a result of cytokine release in response to HSV infection (McFarlane et al., 2019). Although carried out in non-neuronal cells, this study and others (Ulbricht et al., 2012, Kim and Ahn, 2015, Scherer et al., 2016, Chen et al., 2015) suggest that PML itself may contribute to ISG upregulation, so to determine whether PML was indeed required for ISG stimulation in SCG neurons, we carried out RNA sequence analysis in IFNα-treated neurons depleted of PML. Postnatal (P6) murine SCG neurons were transduced with lentiviral vectors expressing non-targeting control or PML-targeting shRNAs (shCtrl and shPML, respectively) and then mock treated or treated with IFNα (600 IU/ml) for 18h prior to RNA extraction.