Lacto-N-Fucopentaose III (LNFPIII) and Lacto-N-Neotetraose (LNnT) Conjugates Induce Differential Changes in the Microbiome of Diet-Induced Obese (DIO) Mice

Gut dysbiosis is a hallmark of obesity and metabolic syndrome (MetS). As a result, numerous studies have focused on altering the microbiome to improve metabolic parameters. Human milk oligosaccharides (HMOs) are present in human colostrum and mature milk and are well-regarded as powerful prebiotics for a breastfeeding infant. In the described studies, we treated diet-induced obese (DIO) mice with HMO conjugates containing 10-12 molecules of lacto-N-fucopentaose III (LNFPIII) or lacto-N-neotetraose (LNnT). We collected fecal samples and performed 16S sequencing to identify microbial alterations as a result of treatment.