Real-time quantitative PCR analysis of Clade A PP2C genes in Brassica juncea var. tumida of different tissues and under multiple stresses. Real-time quantitative PCR analysis of Clade A PP2C genes in Brassica juncea var. tumida of different tissues and under multiple stresses

Clade A PP2C genes in different organs and responding to stresses were screened using qPCR. The BjuACTINs were regarded as house keeping gene.To analyze the gene expression patterns of clade A BjuPP2Cs genes under biotic stress, 14-day-old seedlings were treated with P. brassicae liquid (OD600=0.07) for 0, 0.25, 0.5, 1, 3, 5, 7, and 9 days; to analyze the gene expression patterns of clade A BjuPP2Cs genes under abiotic stresses, 5-day-old seedlings were treated with 50 μM ABA, 100 mM NaCl and 4℃ treatment for 3 h. To analysis the gene expression level in different growth stages of stem swelling, the samples D1 (the stems of 1-month-old seedlings, six leaf stage), D2 (the stems of 2-month-old seedlings, primary stage of stem swelling), D3 (the stems of 3-month-old seedlings, early stage of stem swelling), D4 (the stems of 4.5-month-old seedlings, fast-growing stage of stem swelling) and D5 (the stems of 5-month-old seedlings, last stage of stem swelling) of B. juncea var. tumida which were grown in the field were collected. Overall design: The experiment was designed to gain insight into the temporal and spatial transcription patterns of 20 Clade A PP2C genes in tuber mustard.The experiment was designed to examine the transcription patterns of 20 Clade A PP2C genes in different organs and different stages of stem swollen, as well as response to 50 μM ABA, 100 mM NaCl and 4℃ treatment for 3 h, and response to the pathogen Plasmodiophora brassicae in different time points.