Massive RNA editing in ascetosporean mitochondria

Adenosine-to-inosine(A-to-I) substitution type of RNA editing is mediated by adenosine deaminase acting on RNA (ADAR) and is involved in various essential cellular functions. As ADAR functions in the metazoan nucleus, it may have evolved from adenosine deaminase acting on tRNA in the metazoan ancestor. Although ADAR has not been detected in fungi and early-branching opisthokonts, it has not been explored in other eukaryotic lineages. Here, we detected and analyzed ADAR from two novel rhizarian amoebae and determined that other protists also possess ADAR. This finding indicates that ADAR may have originated from the last common ancestor of eukaryotes (LECA). Furthermore, the ADAR of the rhizarian amoeba is localized in its mitochondria and may be involved in the massive RNA editing in the mitochondria. Such complex RNA editing in the rhizarian amoeba may have a function to mask some lethal mutations in the mitochondrial genomes, which possibly contributes to the acceleration of diversifi..., Sequencing analyses
Approximately 200 ml of mid-exponential phase cell cultures of Paradinida spp. FC901 and SRM-001 were centrifuged at 2,400 × g for 5 min. The cell pellets were frozen and sent to the sequencing company (Azenta, Tokyo, Japan), and the library reconstruction and sequencing analyses were conducted using the default setting. The details of the analyses and the sequence outputs are summarized in Table S4.
The raw fastq data of DNA-seq was divided into 100 subsets using SeqKit, and three subsets of each species were subjected to the contig assembly using SPAdes 3.13 with default settings. From each assembly data, a single possible mitochondrial genomic fragment was detected by BLASTN using the mitochondrial genome sequence of Ophirina amphinema (GenBank accession number: LC369600.1) as the query sequence. The detected sequences were identical among the three subset analyses of each species, while the starting position of each sequence differed. By comparing these sequ..., , # Massive RNA editing in ascetosporean mitochondria

Dataset DOI: [https://doi.org/10.5061/dryad.mcvdnck4z](https://doi.org/10.5061/dryad.mcvdnck4z)

## Description of the data and file structure

The dataset for molecular phylogenetic analysis used in the paper \"*Massive RNA editing in ascetosporean mitochondria*.\"

### Files and variables

#### File: dtst1\_for\_18S

**Description:** The 18S rRNA gene sequences of Paradinida sp. FC901 and SRM-001

#### File: ADAR-ADAT\_allEuk\_4\_FC901-SRM\_strict.trimal.fst

**Description:**  Main dataset for molecular phylogenetic analysis of ADAR-ADAT

## Code/software

It can be viewed with a text editor.

## Access information

Other publicly accessible locations of the data:

* NCBI,